earticle

영어

Bioethanol is the most common renewable fuel today. During pretreatment processes for cellulosic biomass, furan-derived inhibitors such as furfural and hydroxymethylfurfural(HMF) which decrease ethanol productivity are generated. The conversion rate of furfural and HMF is
a key factor for obtaining a high fermentation performance. Enzymes which supply cofactors, or those which convert furfural and HMF to less toxic compounds were chosen as candidate targets. Aldehyde dehydrogenase 6 (ALD6) is involved in supplying a cofactor, NADPH,
which is needed for reduction of furfural and HMF. In addition, the specific rate of furfural oxidation was increased by 2.2-fold higher, and that of HMF oxidation by 2.5-fold higher in the ALD6 overexpressed strain compared to the control strain. Furthermore, ethanol productivity
of the ALD6 overexpressed strain in fermentations with media containing both furfural and HMF increased by 33.2 % compared to the control strain. Consequently, the tolerant properties of S. cerevisiae against furan-derived inhibitors were improved by overproduction of ALD6.