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Since soil is very complex and variable environment, soil-dwelling microorganisms are exposed to many kinds of external compounds. Such compounds may accumulate to toxic levels unless they are metabolized to water- soluble products that can be readily excreted. Cytochrome P450 enzymes often catalyze the initial step in such detoxification pathways. There are multiple forms of cytochrome P450 in a single strain. They are notable for their broad and overlapping
substrate specificities. These enzymes catalyze industrially useful reaction such as hydroxylation, epoxidation etc. A number of studies have been reported that these are differentially expressed by the nducers. In many cases, inducers are also substrates for the induced enzymes. Thus, cytochrome P450 activities remain elevated only as needed. Streptomycetes are one of the most numerous soil bacteria. Among them, we have previously demonstrated that Streptomyces avermitilis is suited for cytochrome P450 reactions of phytochemicals such as isoflavonoid. There are 33 numbers of cytochrome P450 in
Sterptomyces avermitilis. We could observe differential induction of cytochrome P450 by phytochemicals in Streptomyces avermitilis through RT-PCR. These knowledges can provide insights into screening of cytochrome P450 mainly participated in the reaction.