earticle

영어

Previous studies have focused on the AP-1 and CRE motifs located in the near upstream region of the tyrosine hydroxylase (TH) promoter due to their importance in regulating TH gene expression. The composition of both TH AP-1 and CRE DNA-protein complexes in olfactory bulb (OB) were consistent with immunohistochemical results showing that, in odor deprived mice, FosB, but not CRE-binding protein (CREB) and CRE modulator (CREM), binding activities and
protein expression were reduced in parallel with TH levels. In addition, transgenic mice expressing a 9kb TH/lacZ construct with 2 base substitutions in the AP-1 site showed reduced expression of the reporter gene in the OB and not other brain regions. Interestingly, TH activity and immunoreactivity were not reduced in the OB of FosB knockout animals suggesting that another immediate early gene (IEG) may substitute for FosB as a Jun binding partner.