earticle

영어

The main goal of this research is to achieve development of recombinant E. coli for fatty acid synthesis(FAS). Genes for pyruvate dehydrogenase (aceE) and acetyl-CoA carboxylase (accA), which are the enzymes that catalyze the first step in the synthesis of fatty acids in Escherichia coli MG1655, were cloned and characterized. That is, the E. coli strains were midterm recombinant strains to produce fatty acid. The enzyme of aceE gene converts pyruvate to acetyl-CoA and that of accA gene catalyzes the addition of CO2 to acetyl-CoA to generate
malonyl-CoA. The genes were identified as homologous gene of E. coli through a metabolic pathway. The recombinant E. coli MG1655 containing the aceE or accA gene-inserted expression vector(pTrc99A) were constructed. As a result, the intermediate products were analyzed from in vivo metabolites and in vitro metabolites of recombinant E. coli. The two strains produced more malonic acid than its wild type E. coli. However, there was insignificant the difference between E. coli harboring a aceE gene-inserted pTrc99A vector and E. coli harboring a
accA gene-inserted pTrc99A vector.