earticle

논문검색

효소공학

Protein Engineering of Germacredinol Synthase (strep 13) from Streptomyces peucetius ATCC 27952: Characterization of N- and C-Terminal Region of Strep13

원문정보

Deepak UPRETI, Gopal Prasad GHIMIRE, Janardan LAMICHHANE, Jae Kyung SOHNG

한국생물공학회 한국생물공학회 학술대회 2009 추계학술대회 및 국제심포지움 2009.11 pp.173-173
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

The Strep 13 encodes as sesquiterpene alcohol (germacredinol) synthaseencoding from Streptomyces peucetius ATCC27952. Complete open reading frame (ORF) of Strep 13 shows the production of germacredinol/geosmin. Here, we have explored the independent function of N-terminal and C-terminal protein of strep 13. Both terminal recombinant proteins were expressed at high level in Escherichia coli and analysis were carried out. N-terminal protein (~58.15 kDa) showed the Mg2+ dependent activity for the conversion of conversion of Farnesyl diphosphate to Germacrene D. In contrast, the expressed C-terminal domain of Strep13 has no farnesyl diphosphate cyclase activity.

키워드

저자정보

  • Deepak UPRETI Department of Pharmaceutical, Institute of Biomolecule Reconstruction (iBR) ; Department of Biotechnology, Kathmandu University.
  • Gopal Prasad GHIMIRE Department of Pharmaceutical, Institute of Biomolecule Reconstruction (iBR) ; Department of Biotechnology, Kathmandu University.
  • Janardan LAMICHHANE Department of Pharmaceutical, Institute of Biomolecule Reconstruction (iBR) ; Department of Biotechnology, Kathmandu University.
  • Jae Kyung SOHNG Department of Pharmaceutical, Institute of Biomolecule Reconstruction (iBR) ; Department of Biotechnology, Kathmandu University.

참고문헌

자료제공 : 네이버학술정보

    함께 이용한 논문

      ※ 원문제공기관과의 협약기간이 종료되어 열람이 제한될 수 있습니다.

      0개의 논문이 장바구니에 담겼습니다.