earticle

영어

Rapid and quantitative identification of bacterial pathogen has been considered as important criteria for its clinical and hygienical requirements. Among various methods, capillary electrophoresissingle strand conformation polymorphism (CE-SSCP) analysis which
can separate target DNA in sensitive and rapid manner is applied successfully for multiplex quantitative detection of bacteria. However, conventional poly(N,N-dimethylacrylamide) polymer matrix generally used for CE-based analysis has limited resolution in CE-SSCP since it is optimized for molecular weight dependent separation.In this study, multiplex quantitative etection of bacterial pathogens using CE-SSCP was performed with noble high resolution polymer matrix. The marker sequences of targets were amplified with single set of universal PCR primers from conserved region of 16S rRNA gene. PCR products lengths were 238-265bp, and further modification of DNA was not necessary. The results showed that 9-plex quantitative detection was successfully achieved with high resolution and sensitivity, and simple procedure. The results also displayed the potential of new polymer matrix for various genetic analyses.