원문정보
초록
영어
Bacillus sp. stains 2002 and 2003 were isolated from a forest soil samples collected at Taebaek
mountain of Gangwon province, Korea. On the basis of the comprehensive taxonomic data from
morphological, physiological and biochemical characteristics, and 16S rDNA sequence analysis,
BCNU 2002 and 2003 were identified as B. amyloliquefaciens. The in vitro antimicrobial
activities against bacteria such as B. subtilis and M. luteus and pathogenic fungi such as
Aspergillus niger, Candida albicans, Epidermophyton floccosum, Saccharomyces cerevisiae,
Trichophyton mentagrophytes and Trichophyton rubrum were elucidated. The effect of medium
components (i.e. carbon and nitrogen sources) and other culture requirements (i.e. initial pH and
temperature) on production of antimicrobial antibiotics by Bacillus sp. 2002 and Bacillus sp. 2003
were also investigated in this study. Maximum production of bioactive metabolites in a synthetic
medium was found on the seven day of incubation. Furthermore, maintaning the incubation
temperature at 28℃ under shaking condition was found to be optimum for maximum antimicrobial activity. In addition, BCNU 2002 and BCNU 2003 were determined to have the ability to produce enzymes such as amylase, protease, gelatinase and catalase. (Acknowledgments; This research was financially supported by the Korean Ministry of Education, Science Technology
(MEST) and Korea Industrial Technology Foundation (KOTEF) through the Human Resource
Training Project for Regional Innovation)