earticle

영어

Diagnostics and disease-management strategies require technologies to enable the multiplex detection and quantification of a wide range of pathogenic microorganisms. However, most multiplex· quantitative detection methods available suffer from compromises between the
level of multiplexing, throughput and accuracy of quantification. Here, we demonstrate the novel multiplex pathogen detection method using capillary electrophoresis-single strand conformation polymorphism (CE-SSCP) coupled with multiplex ligation-dependent probe amplification
(MLPA). MLPA-CE-SSCP is composed of four major steps which are probe hybridization, probe ligation, multiplex amplification and detection. Using five clinically important pathogens as a model set, all the steps were carefully optimized to precisely quantify by MLPA-CE-SSCP.
We could obtain the results which illustrate a strong potential in clinical diagnosis, food safety, and biosafety.