원문정보
초록
영어
A bacterial flavin-containing monooxygenase (FMO) gene was cloned from Methylophaga aminisulfidivorans MPT, and a plasmid pBlue 1.7 was constructed to express the bacterial fmo gene in E. coli. The recombinant E. coli harboring the pBlue 1.7 plasmid produced 95 mg/L of indirubin and 5 mg/L of indigo in tryptophan medium (2g tryptophan, 10g NaCl, 5g yeast extract per liter) containing several amino acids (cysteine 0.05%, aspratic acid 0.005% and arginine
0.005%, w/v). The aim of this study was the investigation of the optimum indirubin production conditions through batch culture. The optimum conditions were as follows: tryptophan concentration 1g/L, pH 7.0, air supply 1.0 vvm and culture temperature 35℃. Under these
conditions, 100 mg/L indirubin and 4mg/L indigo were produced.