earticle

영어

Laccase, one of the ligninolytic enzymes, received attention from researchers for the past decades due to its broad substrate specificity. Unlike peroxidases, it use molecular oxygen as the final electron acceptor. The potential application of laccase includes pulping, detergents,
textile dyes, biosensors, detoxification of pollution and enzymatic conversion of chemical intermediates. Laccase production from repeated batch cultures of immobilized Pleurotus ostreatus was investigated. Immobilization of P. ostreatus was conducted by polyurethane foam
(PUF). The effect of pore size of PUF was evaluated by laccase activity. Among 25, 40 and 90 pores per inch (PPI), PUF with 40 PPI showed the highest activity in submerged cultures. Replacement of the medium was conducted at different phases of its growth in submerged
fermentation; the exponential, early stationary and death phases. It showed that laccase production of repeated batch culture was significantly affected by the time of replacement of medium.