earticle

영어

The symbiosis of Effective Microorganisms (EM) is investigating. EM consists of eighty different species and generally, there are three main genera of EM which are phototrophic bacteria, lactic acid bacteria, and yeast. Recently, EM is applied in various fields such as in industry,
agriculture and environmental cleanup. Thus, quantitative analysis of EM is necessary for more efficient use because the characteristics and efficacy of EM is depended on distribution ratio of its main genera. Three strains that used in this study are Lactobacillus plantarum, Saccharomyces cerevisiae, and Rhodopseudomonas palustris. Across the study of the cell interaction, co-culture is obviously necessary. Thus, as the beginning stage, we are examining the symbiosis of the EM. The compositions of culture medium and condition are: Glucose
5g l-1, Peptone 4gl-1, NZ-case(casein hydrolysate) 4gl-1, K2HPO4 1g l-1, MgSO4 0.5 l-1, and 32℃ in incubator for 72hr with light up the tungsten lamp. The pH adaptation test on medium is measured by arranging the pH at 3.5-6 separately. Optical density of cell by using Spectrophotometer at 600 nm and plate counting on selective pressure are used to analyze the cell growth. Furthermore, the coexistence of EM is observed by using an optical microscope.