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영어

Pseudomonas sp. BCNU 2001 was isolated from soil samples in Tae-Baek Mountain. The taxonomical position of bacterium was investigated using morphological, biochemical and genetic techniques. These results strongly indicated that it was most closely related to Pseudomonas aeruginosa. The effects of nutrients and influence of environmental parameters on growth and production of bioactive metabolites by Pseudomonas sp. BCNU 2001 were evaluated in submerge culture. Disc diffusion method was employed to study the effect of different culture media, various carbon and nitrogen sources, inoculum size, temperature, pH and incubation period on the production of bioactive metabolite in flask cultures by Pseudomonas sp. BCNU 2001. Growth was determined as dry cell weight in a fixed volume of culture broth. Dextrose, maltose, citrate, mannitol, trehalose, fructose and glycerol were found to be the suitable carbon soures. Especially, BCNU 2001 isolated from Tae-baek mountain was highly active to M. luteus, P. mirabilis and S. cereviea. (Acknowledgments; This research was financially supported by the Korean Ministry of Education, Science Technology (MEST) and Korea Industrial Technology Foundation (KOTEF) through the Human Resource Training Project for Regional Innovation)