earticle

영어

Fumaric acid is the chemical compound with the formula HO2CCH=CHCO2H. This white crystalline compound is one of two isomeric unsaturated dicarboxylic acids, the other being maleic acid wherein the carboxylic acid groups are cis. The commercial demand for fumaric acid has increased because of its extensive applications such as the food industry, the paper industry, the pharmaceutical industry and the feed industry.The purpose of this study is to optimize the main medium for the production of fumaric acid by R. oryzae mutant. Response surface methodology (RSM) was conducted to determine the optimal concentrations of medium
components affecting the production of fumaric acid. The independent variables (Glucose, NZ-amine A, KNO3, CaCO3) were selected on the basis of preliminary experiments. The optimal concentrations of each variable yielding the maximal production of fumaric acid were 7.5% glucose, 0.11% NZ-amine A, 0.11% KNO3 , and 2.6% CaCO3. The maximum value of fumaric acid predicted from the model was 27.4 g/L. Using the optimum medium obtained by RSM, the maximum fumaric acid concentration value achieved in the flask culture was 26.2 g/L, fairly close to the 27.4 g/L predicted by the model. The highest concentration of fumaric acid (32.1
g/L) was produced by R. oryzae in the stirred tank reactor (STR).