earticle

영어

Glucan formed by linking of many glucose molecules has several interesting in properties, and it is structurally divided into two types of α-glucan and ß-glucan depending on the mode of bonding. Among them, ß-glucan is a powerful immune stimulator known to activate macrophages in the immune system. Especially, to enhance production of ß-glucan (exopolysaccharides, EPS), we have optimized media for EPS by Aureobasidium pullulans. The optimization of culture medium was investigated for the production of ß-glucan by statistical experimental design. In the previous study, among organic nitrogen sources, it was found that Ashbya gossypii extract was pivotal factor for the production of ß-glucan. After adaptive nefactor-
at-a-time (OFAT) method experimentation was carried out, the relative importance of medium components for maximum glucan production was evaluated by using Plackett-Burman design (PBD). As the result, it was showed that sucrose, ammonium sulfate and A. gossypii extract were significant components for EPS production. Also, on the basis of PBD analysis, Box-Behnken design and Response Surface Method (RSM) were carried out to search for optimum medium of EPSs productionby using statistical analysis system (SAS) program. Using the optimized parameters from RSM, theoretical maximum production of ß-glucan resulted in
about 60 g/L. Under optimized medium, EPS production of the optimum medium was ten times higher than one (5.34 g/L) of the basal medium.