earticle

영어

It is reported that carotenoid production can be affected by carbon sources used for growing metabolically engineered Escherichia coli expressing heterologous carotenoid pathway enzymes. In order to systematically study the effect of carbon sources on cell growth and
carotenoid formation in metabolically engineered E. coli, six carbon sources (glucose, glycerol, galactose, lactose, mannose and maltose) and four carotenoid structures were chosen. The biosynthetic pathways for four carotenoids, C30 diapolycopene, C30 diapotorulene, C40
lycopene, and C40 beta-carotene, were engineered in E. coli and then the recombinant strains were grown in mineral media supplemented with the carbon sources. Among the six carbon sources, glycerol showed highest performance in the production of all four carotenoids
in E. coli. Worst performance was observed in a medium containing glucose as a carbon source. Although the growth rate and final cell density were similar in media containing carbon sources, the highest carotenoid productivity was obtained in E. coli cells grown on glycerol
as a carbon source.