earticle

영어

Nanofiber mesh has been used as a tissue engineering scaffold that allows the cell-friendly environment due to its three-dimensional extracellular matrix-like structure. Biodegradable synthetic polymers having good mechanical property and easy formulation ability have been used as materials of nanofiber mesh. However, these materials have poor cell adhesion ability and do not include bioactive molecules for ideal tissue engineering scaffolds. Previously, we produced recombinant mussel adhesive protein (MAP), fp-151-RGD, by genetic redesign and showed its great cell adhesion, spreading, and proliferation abilities from coating on tissue culture plate surfaces. In the present work, we fabricated cell-friendly MAP-coated electrospun polycaprolactone (PCL) nanofiber mesh. It was identified that fp-151-RGD was successfully coated on a electrospun PCL nanofiber mesh using scanning electron microscopy and anti-fp-5 antibodystained fluorescence images. Mouse pre-osteoblasts (MC3T3-E1) cells were efficiently attached on MAP-coated nanofiber surfaces compared to non-coated sole PCL nanofiber surfaces. We found that MC3T3-E1 cells were also differentiated efficiently on MAP-coated nanofiber surfaces, demonstrating better mineralization and alkaline phosphatase activity.