earticle

영어

Hybrid mussel adhesive protein (MAP), fp151-RGD, designed and constructed by our research team has excellent adhesion and spreading abilities. Based on its adhesive and cationic properties, fp-151-RGD is possibly suitable for use as a cell-adhesion biomaterial in cell culture
or tissue engineering, and in any other area where efficient cell adhesion is required. In this aspect, we designed oppositely charged polyelectrolyte multilayer (PEM) of fp151-RGD (+) and hyaluronic acid (HA) (-) as a platform surface to improve complication of titanium-based implantation such as aseptic loosening. Each layer was deposited by spin coater, and the built-up of both fp151-RGD and HA layer were confirmed by quartz crystal microbalance and contact angle analyzer. Then, adhesion and proliferation of MC3T3-E1 preosteoblast cells were examined on the multilayer films by direct cell count and MTT methods. As results, we observed that adhesion and proliferation properties of the MC3T3-E1 cells were superior on the
surface-treated titanium to pure titanium foils. Our results suggest that PEM using fp151-RGD and HA can be applied as an efficient coating method for titanium-based implant.