earticle

영어

Photosynthetic microorganisms produce relatively large amounts of physiological activation materials and stimulate the physiological activity of other organisms1). In this study, two mammalian cell were cultured under the different culture medium, Dulbecco’s Modified Eagle medium (DMEM) including fetal bovine serum (FBS) and DMEM including physiological activation materials produced from photosynthetic microorganism. Cell growth was estimated by cell counting in a hemocytometer2). The level of cellular stress was measured using Lyso Tracker dyes, known as a specific fluorescent dye to stain only lysosome3). In comparison with FBS, we did not show significant differences for growth of mammalian cell under the feed condition with physiological activation materials. The growth of the cells can be similarly controlled by varying the photosynthetic microorganism concentration. In addition, we found that any cellular stresses were not induced by adding the physiological activation materials in mammalian cells. Our results suggest that new materials produced from photosynthetic microorganism can enhance the physiological activity of mammalian cells as a nontoxic bioavailable materials.