earticle

영어

WSSV caused major viral diseases in shrimp aquaculture industry. Viral envelop proteins of WSSV play important roles on the virus infection. This study was carried out for the identification of mRNA transcriptional level at various WSSV infection stages using Reverse Transcription-PCR (RT-PCR).mRNAs of shrimps were isolated from shrimp organs at various infection stages after WSSV infection. Through the comparison of mRNA transcriptional levels of infected shrimp organs, it was confirmed that the vaccination of shrimp delayed viral gene transcription for 5~ 10 days. However, the general mRNA transcriptional pattern of vaccinated shrimps showed similar trend to that of unvaccinated shrimps. RT-PCR results from infected shrimps indicated that VP28 was not suitable for the analysis of infection stage due to early and high level transcription in every sample. But, VP26 was a useful marker of infection stage owing to infection stage related
RT-PCR transcriptional pattern. RT-PCR of VP26 could provide information on the shrimp mortality and virus propagation.In further study, infection patterns will be analyzed using mRNA level via RTPCR from infected shrimps using other primer sets.