earticle

영어

3-Hydroxypropionic acid (3-HP), an important platform chemical for several commodity and speciality chemicals was produced in E. coli by over expressing dhaB of Klebsiella pneumoniae DSM 2026 encoding glycerol dehydratase and aldH of E. coli K-12 MG1655 encoding aldehyde dehydrogenase. Adenosylcobalamin, an essential and expensive coenzyme should be provided extrinsically for the activity of DhaB in production of 3-HP in E. coli. To overcome the
limitation of coenzyme B12 in E. coli, the K. pneumoniae strain, which naturally synthesizes 3-hydroxypropionaldehyde (3-HPA), an intermediary compound of glycerol metabolism and serves as the precursor for 3-HP production, was studied. An efficient production of 3-HP in K. pneumoniae required expression of aldH encoding aldehyde dehydrogenase which converts 3-HPA to 3-HP. By over expressing an aldH genes in K. pneumoniae under various cultural
conditions a reasonable titer of 3-HP was achieved. Under microaerobic condition, a maximum titre of 1.44 ± 0.2 gram per litre was successfully produced.