earticle

영어

Sm23, a novel SGNH esterase from Sinorhizobium meliloti 1021 (Sm23), which has conserved amino acid sequences of Gly8(G), Asp9(D), Ser10(S), and Leu11(L) in N-terminal region, is identified and characterized. Enzymatic functions of the enzyme are investigated using activity staining and enantioselective assay with (R,S)-methyl-β- hydroxyisobutyrate, which showed that this enzyme is specific for short chain ester with (R)-specificity. We also studied oligomeric state of Sm23 using chemical cross-linking, high performance liquid chromatography, and mass-tof mass spectrometry which has trimer in native state.