원문정보
초록
영어
3-Hydroxypropionic acid (3-HP) is a potential feedstock for producing many industrially important chemicals. Previously, we reported the production of 3-HP from glycerol by recombinant E. coli SH-BGK1 with a highest titer of 2.8 g/L in shake-flask experiments and 38.7 g /L in bioreactor experiments. The yield was appeared to be about 35%. Most carbons in glycerol were diverted to growth and the production of other metabolites like lactic acid, acetic acid etc. To improve the
yield, glycerol kinase (glpFKX) gene cluster which includes glycerol transport protein were disrupted. Then, for the glycerol transport, glpF gene was cloned and overexpressed under a constitutive tac promoter in a low copy pACYCDuet vector. The resulting construct was grown
in a rich medium and the cells were resuspended and used for resting cell experiment for the production of 3-HP. With the new construct, the yield on glycerol improved to the theoretical maximum of 100%. However, it was necessary to supplement glucose as a carbon source
for maintaining cell viability and enzymatic activities for the 3-HP synthesis from glycerol.