원문정보
초록
영어
ArgR is transcriptional factor in Corynebacterium glutamicum that regulates the ornithine biosynthesis binding to the upstream region of argC, argB, argF, and argG gene. Upon proline supplementation, ArgR binding affinity was significantly reduced upstream of argB resulting in increased ornithine production. Putative transcriptional factor FarR also bound to the upstream of argB gene and it showed inhibitory effect of DNA-binding affinity of ArgR. In addition, this study reports a novel integrated metabolic pathway of amino acid production through the utilization of phenol and naphthalene in C. glutamicum. In the presence of 8.5 mM phenol and 4.2 mM naphthalene, the level of glutamate and proline production was highly increased, compared to the control condition. DNA-binding affinity of ArgR and FarR to the upstream of gltB and gdh gene, which participate glutamate biosynthesis, was reduced significantly in the presence of phenol
and naphthalene. Finally, it suggests that C. glutamicum can apply to the environmentally friendly fertilization technology of aromatic compoundscontaminated soils. [This study was supported by the 21C Frontier Microbial Genomics and Applications Center Program, grant No. 11-2008- 10-002-00, Ministry of Education, Science & Technology, Republic of Korea. The authors are grateful for their support.]