earticle

영어

Bone morphogenetic protein-7 (BMP-7) is a member of the BMP subfamily of the transforming growth factor ß (TGF-ß) superfamily. It induces bone formation and thus renders it to a protein of
pharmaceutical importance. Determination of rhBMP-7 based on ELISA involves many laborious and time - consuming procedures. In this work, immuno-optical sensor has been developed to measure the concentration of rhBMP-7 by sequential injection analysis and to investigate the repeatability and stability with different condition. An optical immuno-sensor was prepared with an optical fiber immobilized with quantum dot (QD)-conjugated antibody. The antibody for the
rhBMP-7 was first conjugated with CdSe/ZnS QDs via EDC/NHS and then immobilized on the tip of an optical fiber with a diameter of 2 mm and a quartz glass plate. The fluorescence intensity changed with the quantity of samples, which were attached to the QD-conjugated antibody.