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For oligonucleotide microarray, target gene is generally amplified by polymerase chain reaction (PCR) and purified. In the present work, we suggest fast and non-labeling direct 16S rRNA detection of pathogenic bacteria without amplification and purification of target gene. Because
16S rRNA has many copies in all living bacteria, we successfully used 16S rRNA as a target for microarray. For simple and fast direct detection of pathogens, we used our previously constructed 16S rDNA-based oligonucleotide microarray detection system. The bacterial
total RNAs from cell lysis were hybridized to a specific 16S rRNA probes and detected using fluorescent-labeled detector probe without labeling reactions of target RNAs. We found that hybridization specificity and sensitivity were enhanced using the fragmented RNAs. We also
investigated the limit of detection (LOD) using Vibrio vulnificus.