원문정보
초록
영어
The bacterial ghost system is a novel vaccine delivery system. It provides versatile carrier functions for foreign antigens with excellent natural intrinsic adjuvant properties. The optimized medium condition for the ghost bacteria, vaccines E. coli K-12/pHCE-InaN-GAPDHGhost27SDM
were determined as 10 g/L glucose, 5 g/L yeast extract and 50 mg/L thiamine with Riesenberg's semi-defined medium according to one-at-a-time method. The optimal batch fermentation
parameters were determined as 10% inoculum size, 300 rpm agitation and 2.0 vvm aeration for the highest production of 3.7 g dcw/L. The nutrient feeding strategy with Riesenberg's defined medium for ghost bacteria vaccine fed-batch process was evaluated. Fed-batch fermentation for the optimized ghost bacteria vaccine production was carried out in 4 phases; batch, fed-batch, induction and heat-shock phases. The heat-shock phase was high temperature holding phase at
47℃ for 2 h to increase ghost cell efficiency. Under the optimal condition of fed-batch fermentation, the cell density of ghost bacteria reached 35 g dcw/L with 99.9% of ghost formation efficiency.