원문정보
초록
영어
This study investigated the production of catalase of Bacillus flexus BKBChE-3. Bacillus flexus BKBChE-3 was screened and identified from soil for the production of catalase. This strain was cultivated in shaking flasks with tryptic soy broth(TSB) complex medium at 30℃
and speed of 200 rpm. Effects of the temperature on the activity of the native catalase and whole cell viability were studied in the temperature range of 25-60℃ and pH range of 2.0-13.0 for stability. Korean natural zeolite was added to shaking flasks and mixed with microorganisms for
immobilization during 24 hours. The native catalase has kept its activity over 50℃. And bacillus cell have survived at extreaming conditions of over 50℃ and pH 11. The hydrogen peroxide was removed at concentration of 10.5 mM within 1 hour.