원문정보
초록
영어
Nitro compounds are relatively rare in nature, with only ~150 known examples, but include synthetic drugs, highly energetic compounds and well known antibiotics such as chloramphenicol and pyrrolnitrin. Especially, biosynthesis of aromatic nitro compounds is poorly
understood, and key enzymes in catalyzing the formation of aromatic nitro group are not characterized well. Here, we present an arylamine N-oxygenase (AurF) through mechanistic and structural studies, which is involved in the biosynthesis of nitro-containing antibiotics including aureothin, SNF4435C/D and spectinabilin. Biosynthetic gene cluster of spectinabilin as an iterative type I polyketide synthase is described and compared with other gene clusters. This study gives some insights of biosynthesis of aromatic nitro compounds for medical and industrial
application.