earticle

영어

Most of the biological mechanisms such as DNA replication, transcription, and gene regulation are based on molecular interactions between DNA and protein (or enzyme). It has been demanded to develop more functional probe molecules, which are useful and proper for analyzing molecule-level study between DNA and protein. In this study, we presented new approaches using DNA oligomer with unnatural nucleobases as novel DNA probes. In particular, nitric oxide (NO)- damaged nucleobases such as oxanine (Oxa) and hypoxanthine (Hyp)
were explored in terms of their biotechnological applications. We prepared Oxa- or Hyp-containing DNA oligomers and employed as novel substrates for DNA-recognizing enzymes or DNA-binding proteins. These methods and the obtained results will be useful for elucidation
of molecular studies based on DNA-protein interactions.