원문정보
초록
영어
Deep eutectic solvents (DES’s) are mixtures of ammonium salts and hydrogen bond donors such as 1:2 choline chloride/urea or choline chloride/glycerol. DES’s are potential green solvents because they are nonvolatile, non-flammable, non-toxic and inexpensive. Many
hydrolases are active in DES’s with rates comparable to those in toluene and up to five-fold higher than rates in typical ionic liquids. The glycerol component of the DES did not compete in the transesterification, indicating that its reactivity in ChCl:Gly is >200- fold lower than expected. Lipase from Candida antarctica was at least 20 times more stable in ChCl:U than in solutions containing the same concentrations of choline chloride and urea. We hypothesize that the
hydrogen-bond network that forms between DES components decreases their reactivity. Addition of 10-25% ChCl:Gly to aqueous solutions enhanced the activity of esterases up to three-fold and of epoxide hydrolase up to 20-fold. Thus, these polar solvents are suitable candidate solvents or cosolvents for biocatalysis.