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영어

High cell density in microbial cell cultivation is about 10 times to a normal density that can be obtained without extraordinary efforts in a batch culture. For instance, if a 5~10g/L of E.coli cell density is considered a normal cell density, 50~100g/L would be called a high cell density. In animal cell culture 106~107/ml cell density would be a high cell density if 105~106 cells/ml. An immobilized cell matrix can have a cell density of 108cells/ml, but the cell density in the reactor
can be lower or much lower than that in the matrix. Extra efforts mean fed-batch, continuous culture, membrane cell recycle for high cell density culture and use of pure oxygen. Several HCDC systems developed at biochemical engineering laboratory of KAIST during the last 30 years will be introduced with a theoretical simulation showing the equivalence of MSC-HCDC with similar titer to fed-batch but with much higher productivities in lactic acid and monoclonal antibody production. Especially depth filter perfusion system (DFPS) for monoclonal antibody production appears to be suitable for MSCHCDC pilot test because of its simplicity in immobilization and no need in separation of cells from the product stream.
MSC-HCDC: multi-stage continuous high cell density culture