earticle

영어

In this presentation, my recent years' interaction with KSBB colleagues will be briefly summarized. Succeeding to my last year's presentation at KSBB spring meeting about biotechnology of medicinal higher fungi, our new progresses in purification of ganoderic acids (antitumor compound) from Ganoderma lucidum and their bioactivity study will be mentioned. Comparative study on individual ganoderic acids (GAs) production and identification of differentially expressed genes between liquid static and shaking cultures of G. lucidum will be mainly demonstrated. The content of individual GAs was higher in liquid static culture than that in conventional shaking culture and the higher production was related to increased transcription of biosynthetic genes. A total of 147 up-regulated ESTs were identified in hyper-production of
GA conditions and this study provided interesting information on possible genes functions related to cell differentiation, GA biosynthesis and its regulation. In addition, enhancement of GAs production and biosynthetic gene expression was achieved by addition of phenobarbital
in liquid static cultures of G. lucidum. The demonstrated molecular biochemical engineering approach will be useful to the industrialization of the antitumor compound production process.