earticle

영어

We are going to present our research achievements on whole-cell biocatalyst-based oxidative biotransformation with oxygenation of cyclohexanone into ε-caprolactone as a model reaction. Cyclohexanone was efficiently converted into ε-caprolactone by recombinant Escherichia
coli expressing the chnB gene encoding cyclohexanone monooxygenase of Acinetobacter calcoaceticus NCIMB9871. ε-Caprolactone was produced to a concentration of 15.3 g/L during fed-batch culture based biotransformation (1). The biotransformation efficiency was significantly improved by using recombinant Corynebacterium glutamicum expressing the same gene. The
biocatalyst allowed product accumulated over 22g/L at the reaction conditions similar to those of recombinant E. coli-based process. The higher product concentration and thus higher productivity seemed to be driven by higher solvent tolerance and cofactor regeneration capacity of C. glutamicum.