earticle

영어

We describe simultaneous assessments of bacterial phenotypes by using an integrated microfluidic system. The microfluidic system enables creating a series of different growth conditions, preserving growth media inside nanoliter reactors, and cultivating multiple bacteria with parallel nanoliter reactors. From a single on chip experiment, we assessed the effect of carbon source compositions on the bacterial phenotypes, i.e. the pattern of growth curves, with a wild Escherichia coli K-12 and lacZ knock-out mutant, as a model bacteria system. Diauxie growth curve, which is the stepwise response of bacteria to mixed carbon sources in a medium, was observed only from wild E. coli K-12. This study provides a new procedure for systematic analyses of phenotypes on effectors from single genotypes or multiple genotypes for microbial technology.