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SMB(Simulated Moving Bed), a continuous chromatography technique, has been used broadly in chemical industries, including for petrochemicals, fine chemicals, pharmaceuticals. Recently, SMB has found active applications in bioindustry, mainly for value-added proteins, such as enzymes, monoclonal antibodies, insulin. It is due to its higher productivity and less consumption of mobile fluid comparing to the conventional batch chromatography in addition to
the general benefits of chromatography of operating at ambient temperature and pressure. SMB, consisting of closed loop of multi chromatography columns, two inlets for feed and eluent streams, and two outlets for streams of separated products, can generate separation of high efficiency by counter-current flowing of mobile and stationary phases with proper operation of valves which connect columns, inlets and outlets. The potency of SMB has been exploited for the
separation of products of biological origin, including organic acids such as L-lactic acid which was produced by the saccharification of paper sludge, and amino acids such as phenylalanine and tryptophan. For the last decade, SMB has been employed for the recovery of sugar alcohols, the separation of fructo-oligosaccharides, and the purification of omega-3 fatty acids such as EPA and DHA with the supports from several industries such as the Wrigley and the CJ Corp. Though being originally designed for binary separation, SMB has been modified for wider applications, such as 2-zone SMB/chromatography hybrid system for the center-cut separation, one-column SMB analogue retrofitting conventional batch chromatography, solvent gradient SMB, and thermally-assisted SMB. SMB operation of size exclusion chromatography was successfully adapted to the continuous refolding of lysozyme, recording first in the world. Currently, rare sugar separation of D-psicose and L-ribose and elimination of endotoxins from antibody products are under study using SMB.