earticle

영어

Trypsin inhibitors and lectins are defense proteins produced by many organisms. From Chinese ‘Large Black Soybeans’, a 60 kDa lectin and a 20 Da trypsin inhibitor (TI) were isolated using chromatography on Q-Sepharose, Mono Q, and Superdex 75. The TI inhibited trypsin and chymotrypsin with an IC50 of 5.7 and 5 μM, respectively. Trypsin inhibitory activity of the TI was stable from pH 3 to 13 and from 0 to 65oC. Hemagglutinating activity of the lectin was stable from pH 2 to 13 and from 0 to 65oC. The TI was inhibited by dithiothreitol, signifying the importance of disulfide bond. The TI and the lectin inhibited HIV-1 reverse transcriptase (IC50=44 and 26 μM), and proliferation of breast cancer cells (IC50=42 and 13.5 μM) and hepatoma cells (IC50=96 and 17 μM). The hemagglutinating activity of the lectin was inhibited most potently by Larabinose. Neither the lectin nor the TI displayed antifungal activity