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논문검색

Effects of Media on the Callogenensis and Cell Mass Production in Cell Cultures of Panax vietnamensis

초록

영어

This study was initiated to investigate the impacts of media types and other components on the callogenensis and cell mass production of Panax vietnamensis in the first step of the cell biomass procedure. Four media were checked: Murashige-Skoog (MS), White, Gamborg and Nitch-All. All the four media were shown potential media for Panax vietnamensis callogenensis and cell mass production, in which the MS medium showed the best results: the successful callogenensis ratio and cell mass formation were 30% and 62,93 ± 3,63 mg (DW) respectively, the Nitch medium showed the lowest results: the successful callogenensis ratio and cell mass formation were 15% and 27,10 ± 2,24 mg (DW) respectively. The results showed that the MS medium is the most suitable medium for Panax vietnamensis callogenensis and cell mass production.

목차

Abstract
 Introduction1)
  Plant material and callus induction
  Cell mass production evaluation
  Growth kinetic observation
  Evaluation of plant growth regulators’ effectson Panax vietnamensis cell mass production
  Statistical analysis
 Results and discussion.
  Effects of media on Panax vietnamensiscallogenensis
  The cell mass changes versus time
  The effects of the plant growth regulators tocell mass production.
  The effects of the NAA concentrations on cellmass productionresults
  The effects of medium pH on the cell massproductionresults.
  The effects of the culture room temperatureon Panax vietnamensis callogenensis
 Discussion

저자정보

  • Hoang Van Luong Vietnam Military Medical University, Hanoi, Vietnam
  • Nguyen Van Long Vietnam Military Medical University, Hanoi, Vietnam
  • Vu Binh Duong Vietnam Military Medical University, Hanoi, Vietnam
  • Nguyen Linh Toan Vietnam Military Medical University, Hanoi, Vietnam
  • Nguyen Van Minh Vietnam Military Medical University, Hanoi, Vietnam
  • Le Bach Quang Vietnam Military Medical University, Hanoi, Vietnam
  • Nam Hyuck Kim Department of Molecular Science & Technology, Graduate School, Ajou
  • Sang Yo Byun Department of Molecular Science & Technology, Graduate School, Ajou University, 3Cosmetic Science Major, Applied Biotech Program, Graduate School, Ajou University

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