earticle

영어

By-products of marine organisms including salmon, skate, flatfish, and yellow goosefish were investigated tosearch for new source of chondroitin sulfate (CS). Agarose gel electrophoresis with chondroitinase depolymerization showedthat purified chondroitin sulfate did not contain any other glycosaminoglycans. 1H-nuclear magnetic resonance (NMR) spectrawere acquired to confirm the structure and purity. The average molecular weight ranging from 22 to 64 kDa was determinedby high performance size exclusion chromatography. Disaccharide compositions and purities were determined by strong anionexchange-high performance liquid chromatography (SAX-HPLC) after chondroitinase ABC depolymerization. SAX-HPLCdata exhibited that the purity was from 81.7±1.3 to 114.2±2.5% and the yield was from 1.3 to 12.5%. All analytical resultsindicate that salmon cartilage, skate cartilage, and yellow goosefish bone could be promising sources of CS to substitute sharkcartilage CS in commercial neutraceuticals.