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Analysis of Transgene Intergration Efficiency into Porcine Fetal Fibroblast using Different Transfection Methods

원문정보

Baek-Chul Kim, Hong-Rye Kim, Myung-Yoon Kim, Chang-Sik Park, Dong-II Jin

한국동물생명공학회(구 한국동물번식학회) Reproductive & developmental biology Volume 33 No 2 2009.06 pp.113-117
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목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Preparation of Porcine Fetal Fibroblast Cells
  Transgenes
  Transfection with a Liposome Reagent
  Transfection by Electroporation
  Detection of the Transgene
  Data Analysis
 RESULTS AND DISCUSSION
  Efficiency of Transfection Liposome Reagents
  Electroporation
  Reselection of Transfected Colonies
 REFERENCES

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저자정보

  • Baek-Chul Kim Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam NationaI University
  • Hong-Rye Kim Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam NationaI University
  • Myung-Yoon Kim Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam NationaI University
  • Chang-Sik Park Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam NationaI University
  • Dong-II Jin Division of Animal Science & Resources, Research Center for Transgenic and Cloned Pigs, Chungnam NationaI University

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