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Effects of FBS(Feta1 Bovine Serum) and pFF(Porcine Follicular Fluid) on In Vitro Maturation and Development of Porcine Parthenogenetic and Nuclear Transfer Embryos

원문정보

Hyo-Jin Moon, Joo-Hyun Shim, In-Sun Hwang, Mi-Rung Park, Dong-Hoon Kim, Yeoung-Gyu Ko, Choon-Keun Park, Gi-Sun Im

한국동물생명공학회(구 한국동물번식학회) Reproductive & developmental biology Volume 33 No 2 2009.06 pp.85-91
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목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Preparation of Porcine Follicular Fluid (pFF)
  Collection of Oocytes and In Vitro Maturation (IVM)
  Production of Parthenogenetic (PA) Embryos
  Brilliant Cresyl Blue Test (BCB)
  Cdc2 Kinase Assay
  Apoptosis Assays
  Preparation of Porcine Fetal Fibroblast Cells
  Production of Nuclear Transfer (NT) Embryos
  Statistical Analysis
 RESULTS
  Effect of FBS and pFF on In Vitro Maturation and In Vitro Development of Porcine PA and NT Embryos
  BCB Staining and Cdc2 Kinase Activity of the Oo- cytes Matured in Different Condition
 DISCUSSION
 REFERENCES

키워드

저자정보

  • Hyo-Jin Moon Division of Animal Biotechnology, National Institute of Animal Science, RDA
  • Joo-Hyun Shim Division of Animal Biotechnology, National Institute of Animal Science, RDA
  • In-Sun Hwang Division of Animal Biotechnology, National Institute of Animal Science, RDA
  • Mi-Rung Park Division of Animal Biotechnology, National Institute of Animal Science, RDA
  • Dong-Hoon Kim Division of Animal Biotechnology, National Institute of Animal Science, RDA
  • Yeoung-Gyu Ko Division of Animal Biotechnology, National Institute of Animal Science, RDA
  • Choon-Keun Park College of Animal Science, Kangwon National University
  • Gi-Sun Im Division of Animal Biotechnology, National Institute of Animal Science, RDA

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