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Establishment of Baculovirus Infected Insect Cell Line Expressing Porcine Salivary Lipocalin(SALl) Protein

원문정보

HeeWon Seo, Da-Young Park, Mingoo Kim, Mi-Hyun Ahn, Kinarm Ko, Kisung Ko, Hakhyun Ka

한국동물생명공학회(구 한국동물번식학회) Reproductive & developmental biology Volume 33 No 2 2009.06 pp.71-76
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목차

ABSTRACT
 INTRODUCTION
 MATERIALS AND METHODS
  Porcine SALl cDNA Cloning
  Construction of Expression Plasmid
  Formation of Bacmid DNA
  Insect Cell Culture and Transfection
  Virus Titration and Infection to Insect Cells
  Protein Expression of SALl Virus Infected Insect Cells
 RESULTS
  Confirmation of the Expression SAL1-6His Gene in Bacmid
  Expression of SALl-6His Protein in insect Cells
 DISCUSSION
 REFERENCES

키워드

저자정보

  • HeeWon Seo Division of BiologicaI Science and Technology, and Institute of Biomateriah, Yonsei University
  • Da-Young Park Department of Biological Science, Wonkwang University
  • Mingoo Kim Division of BiologicaI Science and Technology, and Institute of Biomateriah, Yonsei University
  • Mi-Hyun Ahn Department of Biological Science, Wonkwang University
  • Kinarm Ko Department of Cell and DevehpmentaI Biology, Max Planck Institute for Molecular Biomedicine, Muenster, Germany
  • Kisung Ko Department of Biological Science, Wonkwang University
  • Hakhyun Ka Division of BiologicaI Science and Technology, and Institute of Biomateriah, Yonsei University

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