earticle

영어

Gamma (γ)-irradiation can be used to control pathogens such as Vibrio vulnificus in seafood. The effects ofirradiation on microbial cell populations (%) have been studied in order to develop detection methods for irradiated foods. Themethod used in this study was ethidium bromide monoazide (EMA) real-time polymerase chain reaction (PCR), using V.vulnificus specific primer, EMA, and SYBR® Green to discriminate between γ-irradiated and non-irradiated cells. Confocalmicroscope examination showed that γ-irradiation damaged portions of the cell membrane, allowing EMA to penetrate cells ofirradidated V. vulnificus. γ-Irradiation at 1.08 KGy resulted in log reduction (−1.15±0.13 log reduction) in genomic targetsderived from EMA real-time PCR. The combination cold/heat shock resulted in the highest (−1.74±0.1 log reduction)discrimination of dead irradiated V. vulnificus by EMA real-time PCR.