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논문검색

Expression of Cyclomaltodextrinase Gene from Bacillus halodurans C-125 and Characterization of Its Multisubstrate Specificity

원문정보

Hye-Jeong Kang, Chang-Ku Jeong, Myoung-Uoon Jang, Seung-Ho Choi, Tae-Jip Kim, Min-Hong Kim, Jun-Bae Ahn, Sang-Hwa Lee, Sook-Ja Jo

한국식품과학회 Food Science and Biotechnology Volume 18 Number 3 2009.06 pp.776-781
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초록

영어

A putative cyclomaltodextrinase (BHCD) gene was found from the genome of Bacillus halodurans C-125, whichencodes 578 amino acids with a predicted molecular mass of 67,279 Da. It shares 42-59% of amino acid sequence identity withcommon cyclomaltodextrinase (CDase)-family enzymes. The corresponding gene was cloned by polymerase chain reaction(PCR) and the dimeric enzyme with C-terminal 6-histidines was successfully overproduced and purified from recombinantEscherichia coli. BHCD showed the highest activity against β-CD at pH 7.0 and 50oC. Due to its versatile hydrolysis andtransglycosylation activities, BHCD has been confirmed as a member of CDases. However, BHCD can be distinguished fromother typical CDases on the basis of its novel multisubstrate specificity. While typical CDases have over 10 times higher activityon β-CD than starch or pullulan, the CD-hydrolyzing activity of BHCD is only 2.3 times higher than pullulan. In particular, itshowed significantly higher activity ratio of maltotriose to acarbose than other common CDase-family enzymes.

목차

Abstract
 Introduction
 Materials and Methods
 Results and Discussion
 References

키워드

저자정보

  • Hye-Jeong Kang Department of Food Science and Technology, Chungbuk National University, Cheongju, Chungbuk 361-763, Korea
  • Chang-Ku Jeong Department of Food Science and Technology, Chungbuk National University, Cheongju, Chungbuk 361-763, Korea
  • Myoung-Uoon Jang Department of Food Science and Technology, Chungbuk National University, Cheongju, Chungbuk 361-763, Korea
  • Seung-Ho Choi Department of Food Science and Technology, Chungbuk National University, Cheongju, Chungbuk 361-763, Korea
  • Min-Hong Kim MH2 Biochemical Co., Ltd., Eumseong, Chungbuk 369-841, Korea
  • Jun-Bae Ahn Department of Food Service Industry, Seowon University, Cheongju, Chungbuk 361-741, Korea
  • Sang-Hwa Lee Department of Food and Nutrition, Seowon University, Cheongju, Chungbuk 361-741, Korea
  • Sook-Ja Jo Department of Food and Nutrition, Seowon University, Cheongju, Chungbuk 361-741, Korea
  • Tae-Jip Kim Department of Food Science and Technology, Chungbuk National University, Cheongju, Chungbuk 361-763, Korea

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