earticle

영어

Lysozyme was treated with digestive enzymes and the production of interleukin 8 (IL-8) was measured in Caco-2cell with the peptides from lysozyme upon stimulating with lipopolysaccharide (LPS) to investigate the overall anti-inflammatory activity of lysozyme when it is in digestive tracts. Lysozyme reduced IL-8 production, and the peptides frompepsin hydrolysis of lysozyme had the similar effect. The products of trypsin digestion of lysozyme had no effect on thereduction of IL-8 production while those of pepsin-trypsin hydrolysis did. The effectiveness of lowering IL-8 production wasnot different by time of the peptide addition. When Caco-2 cells were pre-incubated with peptides for 24 hr, the reductioneffects were observed from the peptides from pepsin hydrolysis, indicating that some of the peptides are still remaining in thecells. Therefore, it can be concluded that the IL-8 reduction effect of lysozyme against LPS still remained even after thepepsin and trypsin hydrolysis.