earticle

영어

A multiplex polymerase chain reaction (PCR) method was developed for the detection of 4 events of geneticallymodified (GM) soybean. The event-specific primers were designed from 4 events of GM soybean (RRS, A2704-12,DP356043-5, and MON89788). The lectin was used as an endogenous reference gene of soybean in the PCR detection. Theprimer pair YjLec-4-F/R producing 100bp amplicon was used to amplify the lectin gene and no amplified product wasobserved in any of the 9 different plants used as templates. This multiplex PCR method allowed for the detection of event-specific targets in a genomic DNA mixture of up to 1% GM soybean mixture containing RRS, A2704-12, DP356043-5, andMON89788. In this study, 20 soybean products obtained from commercial food markets were analyzed by the multiplex PCR.As a result, 6 samples contained RRS. These results indicate that this multiplex PCR method could be a useful tool formonitoring GM soybean.