earticle

영어

Germination is well-known to enhance the digestibility, functionality, and palatability of plant seeds. To examinethe functionality of germinated-safflower seed (GSS), proliferative and differentiative effects of GSS extract on the mousecalvarial bone cells were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolinbromide (MTT) assay and alkalinephosphatase activity, respectively. Water extract of GSS increased dose-dependently proliferative and differentiative effects oncalvarial bone cell, and its effects were stronger than those of ungerminated-safflower seeds (UGSS) extract. One majorcomponent was isolated from GSS extract by a series of purification procedure of solvent fractionation, Diaion HP-20, andSephadex LH-20 column chromatographies. Its chemical structure was identified as trachelogenin (TC) by nuclear magneticresonance (NMR) and mass spectrometry (MS) spectral analysis. Trachelogenin showed significant proliferative (125.7%) anddifferentiative (132.1%) effects on calvarial bone cells at 10−8 M, and its effects were significantly higher than those of 17β-estradiol (E2). TC was found to be a major active compound responsible for high proliferative and differentative effects of thewater extract of GSS. Therefore, these results suggest that TC in GSS may be useful as potential therapeutic agent for theprevention and treatment of bone loss.