earticle

영어

Extract of water-soluble polysaccharide (CFWx), showing inhibiting activity on α-glucosidase, was prepared fromthe fruiting bodies of Cordyceps militaris by hot-water extraction, and ethanol precipitation. Chemical characteristics of CFWxwere as follows: carbohydrate content 30% including 16% of uronic acid; 51% protein content; monosaccharide composition,Man:Glu:Gal (30:43:27); molecular weight 3-5×104. CFWx was further purified by ion-exchange, gel-permeation, and affinitychromatography and CFWx-AH-α fraction was isolated. Fundamental structure of CFWx-AH-α was deduced as α-(1→4)-D-glucan with α-(1→3)- and/or α-(1→6)-D-glycosidic side chains based on methylation analysis.