earticle

영어

A one-step simultaneous immunchromatographic (OS-ICG) assay using colloidal gold-monoclonal antibody(gold-MAb) conjugates was developed for the rapid multianalysis of aflatoxin B1 (AFB1) and ochratoxin A (OTA) in feedsamples. Visual detection limits for AFB1 and OTA were 0.5 and 2.5ng/mL, respectively, and the results were obtainedwithin 15min. Matrix interference from the feed extracts was efficiently reduced by appropriate dilution with buffer. Cut-offvalues of the OS-ICG assay for the feed spiked with AFB1/OTA mixtures (5/5, 10/10, 25/25, 50/55, 100/100µg/kg) were 10and 50µg/kg for AFB1 and OTA. The comparative analyses of 65 feed samples by OS-ICG, enzyme-linked immunosorbentassay (ELISA), and high performance liquid chromatography (HPLC) showed good agreement. In this study, we confirmedthat simultaneous analysis based on immunoassay is possible and it can be used as an on-site multianalysis of AFB1 and OTAin feed, food, and agricultural products.