활성산소계가 돼지 정자의 기능에 미치는 영향


Effects of Reactive Oxygen Species on the Function of Porcine Spermatozoa

김병각, 김기중, 이용안, 김방진, 김용희, 류범용

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The current study was designed to evaluate the effects of the reactive oxygen species (ROS) generated with a xanthine (X) and xanthine oxidase system (XO) on sperm function and DNA fragmentation in porcine spermatozoa. ROS were produced by a combination of 1,000 μM X and 50 mU/ml XO. The ROS scavengers such as superoxide dismutase (SOD) (200 U/ml) and catalase (CAT) (500 U/ml) were also tested. Spermatozoa were incubated for 2 hours in BWW medium with a combination of X-XO supplemented with or without antioxidants at 37℃ under 5% CO2 incubator. Ca-ionophore-induced acrosome reaction, the proportion of swollen spermatozoa under hypo-osmotic condition, malondialdehyde formation for the analysis of lipid peroxidation, and the proportion of DNA fragmentation were determined after 2 hours incubation. The action of ROS on porcine spermatozoa resulted in decreased Ca-ionophore-induced acrosome reaction and membrane integrity, increased the formation of malondialdehyde, and the proportion of sperm with DNA fragmentation(p<0.05). The toxic effects caused by ROS were completely alleviated by CAT in terms of sperm function and characteristics, however SOD did not serve the same scavenger effect as CAT. To conclude, the ROS can cause significant damage to porcine sperm functions and characteristics, which can be minimized by the use of antioxidants.


 재료 및 방법
  정자의 침체반응 분석
  정자의 저장액 처리(Hypo-osmotic swelling test; HOST)
  정자 막 지방의 산화(Lipid Peroxidation)
  유세포 분석기(Flow Cytometry)를 이용한 정자 DNA Frag-mentation 분석


  • 김병각 Byung-Gak Kim. 중앙대학교 동물자원과학과
  • 김기중 Ki-Jung Kim. 중앙대학교 동물자원과학과
  • 이용안 Yong-An Lee. 중앙대학교 동물자원과학과
  • 김방진 Bang-Jin Kim. 중앙대학교 동물자원과학과
  • 김용희 Yong-Hee Kim. 중앙대학교 동물자원과학과
  • 류범용 Buom-Yong Ryu. 중앙대학교 동물자원과학과


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