원문정보
초록
영어
Placenta is the main nutrition source for the fetus during pregnancy. Thus, it has a pivotal function in the pregnant process. Many functions of the placenta have been elucidated. An abnormal placenta is associated with a high rate of pregnancy failure in somatic cloned bovine. Differentially expressed genes (DEGs) were examined in a comparison between normal and cloned bovine placenta using annealing control primer (ACP)-based GeneFishing PCR. Using 120 ACPs, nearly 80 genes were identified and the fragments of 42 DEGs were sequenced. 38 of these genes were known genes and four were unknown. To determine the DEGs result, six target clones expressing on one-side of a normal and a clone placenta were selected. Through an analysis of the target genes using the real-time PCR, the expressing pattern was found to be somewhat different from the DEGs. Additionally, several genes appeared with the same expression pattern. Taken together, this suggests that the target genes would be essential for research into what influences the placental formative mechanisms during fetal development.
목차
INTRODUCTION
MAERIALS AND METHODS
Source for Nuclear Transferred Somatic Cells and Nuclear Transfer
Generation of Placental Tissues
RNA Isolation
cDNA Synthesis and ACP RT-PCR Analysis
Cloning and Sequencing
Semi-Quantitative RT-PCR
Real-Time PCR
Statistical Analysis
RESULTS
Analysis of DEGs in Cloned Bovine Placenta
Semi-Quantitative RT-PCR
Real-Time PCR
DISCUSSION
REFERENCES